Micrococcal Nuclease from Cell Signaling Technology

Product Specs
ItemMicrococcal Nuclease
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number10011S
Quantity60 µl
ApplicationsChIP
Molecule NameMicrococcal Nuclease
Research AreaRelated Products

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Histone deacetylase 3 regulates microglial function through histone deacetylationEpigeneticsDecember 31, 2023Laura Meleady, Morgan Towriss, Jennifer Kim, Vince Bacarac, Vivien Dang, Megan E. Rowland, Annie Vogel Cierniatreatment condition for whole cell chromatin using micrococcal nuclease (Cell Signaling Technology #10011) digestion to mononucleosomes. qPCR of input samples was run for each qPCR primer set in serial dilutions

(2) Low level of ARID1A contributes to adaptive immune resistance and sensitizes triple‐negative breast cancer to immune checkpoint inhibitorsCancer CommunicationsSeptember 1, 2023Xin‐Yu Chen, Bin Li, Ye Wang, Juan Jin, Yu Yang, Lei‐Huan Huang, Meng‐Di Yang, Jian Zhang, Bi‐Yun Wang, Zhi‐Ming Shao, et al.approximately 1 × 107 and cross‐linked by 1% formaldehyde. Chromatin was digested with micrococcal nuclease (10011, Cell Signaling Technology), and the nuclear membrane was broken through several pulses. The DNA fragment

(3) MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongationNucleic acids researchAugust 25, 2023Jin B, Zhu J, Pan T, Yang Y, Liang L, Zhou Y, Zhang T, Teng Y, Wang Z, Wang X, et al.centrifugation at 2000 g and digested in Buffer B containing 25 U of micrococcal nuclease (MNase) (10011, CST) per immunoprecipitation (IP) for 20 min at 37°C, followed by pulsed ultrasonication to shear cellular

(4) Phosphorylation of Neurofilament Light Chain in the VLO Is Correlated with Morphine-Induced Behavioral Sensitization in RatsInternational Journal of Molecular SciencesApril 22, 2023Yu-Xiang Zhang, Yuan-Mei Zhu, Xi-Xi Yang, Fei-Fei Gao, Jie Chen, Dong-Yu Yu, Jing-Qi Gao, Zhen-Nan Chen, Jing-Si Yang, Chun-Xia Yan, et al.supernatants and continue with chromatin digestion. Chromatin was digested with micrococcal nuclease (#10011, Cell Signaling Technology) for 20 min at 37 °C with frequent mixing to digest DNA into a length of

(5) Loss of Kmt2c in vivo leads to EMT, mitochondrial dysfunction and improved response to lapatinib in breast cancerCellular and Molecular Life SciencesApril 1, 2023Nikiana Simigdala, Anna Chalari, Aimilia D. Sklirou, Evangelia Chavdoula, George Papafotiou, Pelagia Melissa, Aimilia Kafalidou, Nikolaos Paschalidis, Ioannis S. Pateras, Emmanouil Athanasiadis, et al.chromatin immunoprecipitation as previously described [29] with an additional step of micrococcal nuclease incubation (CST #10011) prior to DNA sonication with metal probe. Antibodies: H3K4me1 (CST #5326), H3K4me3 (CST #9751), H3K27ac

(6) The MYC-regulated lncRNA LNROP (ENSG00000254887) enables MYC-driven cell proliferation by controlling the expression of OCT2Cell Death & DiseaseFebruary 27, 2023Daniel García-Caballero, Jonathan R. Hart, Peter K. Vogtfragmentation was performed enzymatically by adding 0.5 µL of Micrococcal Nuclease (Cell Signaling Technology; #10011) and incubation at 37 °C for 20 minutes to obtain the appropriate length of DNA fragments. ChIP was

(7) PTBP2-Mediated Alternative Splicing of IRF9 Controls Tumor-Associated Monocyte/Macrophage Chemotaxis and Repolarization in Neuroblastoma ProgressionResearch (Washington, D.C.)January 1, 2023Tang J, He J, Guo H, Lin H, Li M, Yang T, Wang HY, Li D, Liu J, Li L, et al.the reaction was stopped by 4 μl of 0.5 M EDTA. Total RNA was fragmented by micrococcal nuclease (#10011, Cell Signaling Technology, Beverly, USA) digestion, while the input RNA was separated by electrophoresis

(8) IFNγ-dependent silencing of TFF1 during Helicobacter pylori infectionOpen BiologyDecember 14, 2022D. Eletto, F. Mentucci, M. Vllahu, A. Voli, A. Petrella, F. Boccellato, T. F. Meyer, A. Porta, A. Toscowere treated for nuclei extraction and DNA shearing with Micrococcal Nuclease (Cell Signaling, no. 10011, USA) for 20 min at 37°C to obtain DNA fragments of approximately 150–900 bp. Nuclei were sonicated

(9) Histone macroH2A1 is a stronger regulator of hippocampal transcription and memory than macroH2A2 in miceCommunications BiologyDecember 1, 2022Gurdeep Singh et al.10 s, 30 s rest between sonications) before digestion with micrococcal nuclease (Cell Signaling Cat#10011) for 20 min at 37 °C. Lysate was centrifuged at 17 000 RCF for 5 min at 4 °C, aliquoted and diluted

(10) BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiencyNature communicationsOctober 5, 2022Wang F, Liu Y, Ni F, Jin J, Wu Y, Huang Y, Ye X, Shen X, Ying Y, Chen J, et al.Tissues were isolated into single cell suspensions using a Dounce homogenizer. Micrococcal Nuclease (10011, Cell Signaling) was added into each immunoprecipitation (IP) preparation, and the contents were mixed

(11) MiR-585-5p impedes gastric cancer proliferation and metastasis by orchestrating the interactions among CREB1, MAPK1 and MITFFrontiers in ImmunologyOctober 4, 2022Yunwei Wang, Ming Li, Jiaoxia Zeng, Yunshu Yang, Zengshan Li, Sijun Hu, Fangfang Yang, Na Wang, Wenlan Wang, Jun TieDDT (dithiothreitol) in line with kit instructions. Micrococcal Nuclease (Cell Signaling Technology, #10011) was used for digesting DNA into fragments of 150-900 bp. Following ultrasonication, the supernatants

(12) Transcription factor Creb3l1 maintains proteostasis in neuroendocrine cellsMolecular MetabolismSeptember 1, 2022Mingkwan Greenwood, Benjamin T. Gillard, Rizwan Farrukh, Alex Paterson, Ferdinand Althammer, Valery Grinevich, David Murphy, Michael P. Greenwoodresuspended in 500 μl of buffer B. To this sample 3.75 μl of Micrococcal Nuclease (Cell Signaling Technology, 10011) was added and incubated at 37 °C for 20 min in a water bath to digest the DNA, mixing by inversion

(13) Modulation of Breast Cancer Cell FASN Expression by Obesity-Related Systemic FactorsBreast Cancer: Basic and Clinical ResearchAugust 22, 2022Bryan McClellan, Tommy Pham, Brittany Harlow, Gabby Lee, Duan Quach, Christopher Jolly, Andrew Brenner, Linda deGraffenrieddigestion with a micrococcal nuclease enzyme per manufacturer’s instructions (Cell Signaling Technologies, #10011). Chromatin was sheared using a VirSonic 100 ultrasonic sonicator 3× 20 seconds on ice. The resulting

(14) Targeting HECTD3-IKKα axis inhibits inflammation-related metastasisSignal transduction and targeted therapyAugust 3, 2022Li F, Liang H, You H, Xiao J, Xia H, Chen X, Huang M, Cheng Z, Yang C, Liu W, et al.collected by centrifuge and resuspended in 100 μL cytoplasmic lysis buffer with micrococcal nuclease (CST, #10011) to digest genome DNA at 37 °C for 30 min. The pellet nuclei were collected again and resuspended in

(15) Loss of NFE2L3 protects against inflammation-induced colorectal cancer through modulation of the tumor microenvironmentOncogeneMarch 10, 2022James Saliba et al.Subsequently, DNA was lysed for 20 min at 37 °C using 0.4 μL of micrococcal nuclease (Cell Signaling, 10011). Digestion was stopped by adding 10 μL of 0.5 mol/L EDTA (Cell Signaling, 7011) per IP prep on ice

(16) SARS-CoV-2 viroporin encoded by ORF3a triggers the NLRP3 inflammatory pathwayVirologyMarch 1, 2022Xu H, Akinyemi IA, Chitre SA, Loeb JC, Lednicky JA, McIntosh MT, Bhaduri-McIntosh S.Technology, Cat. 7007), and then treated with 0.5 μL of micrococcal nuclease (Cell Signaling Technology, Cat. 10011) for 20 min at 37 °C. Nuclei were resuspended in 1 × ChIP buffer (Cell Signaling Technology, Cat. 7008)

(17) The histone chaperone Anp32e regulates memory formation, transcription, and dendritic morphology by regulating steady-state H2A.Z binding in neuronsCell ReportsAugust 17, 2021Gilda Stefanelli et al.400ul of TM2 buffer. Nuclei were pre warmed at 37°C for 5 min and MNase (Cell Signaling Technologies 10011S) was added to a final concentration of 1.25 U/ml for 10 min at 37°C. EGTA to a final concentration of

(18) Genome-Wide Role of HSF1 in Transcriptional Regulation of Desiccation Tolerance in the Anhydrobiotic Cell Line, Pv11International Journal of Molecular SciencesMay 28, 2021Shoko Tokumoto et al.chromatin digestion. To digest DNA to a length of approximately 150–300 bp, 2 µL micrococcal nuclease (Cat#10011 by Cell Signaling Technology) was added and the samples were incubated for 20 min at 37 ◦ C. After terminating

(19) Genome-Wide Chromatin Analysis of FFPE Tissues Using a Dual-Arm Robot with Clinical PotentialCancers (Basel)April 28, 2021Syuzo Kaneko et al.100 µL of Buffer B with 0.5 mM DTT. Then, 1 µL of micrococcal nuclease (Cell Signaling Technology, #10011) was added into the nuclei mixture and incubated for 20 min at 37 ◦ C with frequent mixing (800 rpm).

(20) M-Cadherin Is a PAX3 Target During Myotome PatterningFrontiers in Cell and Developmental BiologyApril 1, 2021Joana Esteves de Lima, Reem Bou Akar, Myriam Mansour, Didier Rocancourt, Margaret Buckingham, FrÃ©dÃ©ric Relaixincubated for 15 min on ice. Chromatin digestion was performed with Micrococcal nuclease (Cell Signaling, #10011) for 40 min at 37°C. The supernatant containing 20 μg of DNA was incubated with 20 μL of magnetic beads

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Micrococcal Nuclease from Cell Signaling Technology

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Micrococcal Nuclease from Cell Signaling Technology

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